[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2020-4-10] [發(fā)布人:] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Catenococcus Agar

培養(yǎng)基配方
Composition per 1001.0mL:
Agar, noble...............................................20.0g
NaCl......................................................20.0g
K2HPO4 ...................................................5.54g
NH4Cl .....................................................0.5g
MgSO4 ·7H2O...............................................0.3g
KH2PO4 ...................................................1.84g
Sodium acetate............................................0.82g
CaCl2 ·2H2O...............................................0.1g
Yeast extract.............................................0.05g
Trace elements solution SL-6 .............................1.0mL
pH 6.8 ± 0.2 at 25°C
Trace Elements Solution SL-6:
Composition per liter:
MnCl2 ·4H2O...............................................0.5g
H3BO3 .....................................................0.3g
CoCl2 ·6H2O ..............................................0.2g
ZnSO4 ·7H2O...............................................0.1g
Na2MoO4 ·2H2O ...........................................0.03g
NiCl2 ·6H2O..............................................0.02g
CuCl2 ·2H2O .............................................0.01g
Preparation of Trace Elements Solution SL-6 : Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Adjust pH to 6.8. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or
leave in tubes.
Use: For the cultivation of Catenococcus thiocyclus.