[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2020-4-10] [發(fā)布人:] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Catenococcus Medium

培養(yǎng)基配方

Composition per 1003.0mL:
Agar .....................................................15.0g
NaCl......................................................15.0g
K2HPO4 ....................................................1.0g
NH4Cl .....................................................0.5g
KH2PO4 ...................................................0.15g
CaCl2 ·2H2O solution....................................10.0mL
MgSO4 ·7H2O solution....................................10.0mL
Sodium acetate solution..................................10.0mL
Yeast extract solution....................................2.0mL
Trace elements solution SL-4 .............................1.0mL
pH 7.0 ± 0.2 at 25°C
CaCl2 ·2H2O Solution:
Composition per 10.0mL:
CaCl2 ·2H2O...............................................0.1g
Preparation of CaCl2 ·2H2O Solution:  Add CaCl2 ·2H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
MgSO4 ·7H2O Solution:
Composition per 10.0mL:
MgSO4 ·7H2O................................................1.0g
Preparation of MgSO4 ·7H2O Solution:  Add MgSO4 ·7H2O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C.
Sodium Acetate Solution:
Composition per 10.0mL:
Sodium acetate.............................................0.82g
Preparation of Sodium Acetate Solution:  Add sodium acetate to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C.
Yeast Extract Solution:
Composition per 10.0mL:
Yeast extract...............................................1.0g
Preparation of Yeast Extract Solution:  Add yeast extract to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Trace Elements Solution SL-4:
Composition per liter:
EDTA...........................................................0.5g
FeSO4 ·7H2O...................................................0.2g
Trace elements solution SL-6................................100.0mL
Trace Elements Solution SL-6:
Composition per liter:
MnCl2 ·4H2O ...................................................0.5g
H3BO3 ..........................................................0.3g
CoCl2 ·6H2O....................................................0.2g
ZnSO4 ·7H2O....................................................0.1g
Na2MoO4 ·2H2O.................................................0.03g
NiCl2 ·6H2O...................................................0.02g
CuCl2 ·2H2O...................................................0.01g
Preparation of Trace Elements Solution SL-6 : Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Trace Elements Solution SL-4:  Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except CaCl2 ·2H2O so-
lution, MgSO4 ·7H2O solution, sodium acetate solution, yeast extract
solution, and trace elements solution SL-4, to distilled/deionized water
and bring volume to 970.0mL. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°–55°C. Aseptically add 10.0mL of sterile CaCl2 ·2H2O solution,
10.0mL of sterile MgSO4 ·7H2O solution, 10.0mL of sterile sodium ac-
etate solution, 2.0mL of sterile yeast extract solution, and 1.0mL of
sterile trace elements solution SL-4. Mix thoroughly. Pour into sterile
Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Catenococcus thiocyclus.